GeneTex
United States (US)

UBE2B antibody

GTX100416_40366_20170323_WB_M_brain_19011506_488.jpg
GTX100416_40366_WB_B_19011506_597.jpg
GTX100416_39384_IFA_19011506_883.jpg

Cat No. GTX100416

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

mouse brain , 3xFlag-human UBE2B-transfected 293T

Predict Reactivity

Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.72 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Conjugating Enzyme E2 B,E2-17Kda,Hhr6B,Hr6B,Rad6B,Ubc2,Ube2B

Cellular Localization

Cell membrane , Nucleus

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100416_40366_20170323_WB_M_brain_19011506_488.jpg

GTX100416 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX100416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX100416_40366_WB_B_19011506_597.jpg

GTX100416 WB Image

UBE2B antibody detects UBE2B protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of 3xFlag-human UBE2B-transfected 293T cells
12 % SDS-PAGE
UBE2B antibody (GTX100416) dilution: 1:5000

GTX100416_39384_IFA_19011506_883.jpg

GTX100416 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UBE2B(GTX100416) antibody at 1:500 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf