Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX114867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX114867) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.