*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
mouse brain , DDDDK-tagged UBE2B-transfected 293T
Rat, Zebrafish, Rabbit, Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1.02 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the center region of human UBE2B. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Ubiquitin Conjugating Enzyme E2 B,E2-17Kda,Hhr6B,Hr6B,Rad6B,Ubc2,Ube2B
Cell membrane , Nucleus
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq]
GTX114867 WB Image
Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX114867) diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.
GTX114867 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2B antibody (GTX114867) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.