GeneTex
United States (US)

UBE2B antibody

GTX16443_20180530_IHC-P_1_18121410_576.jpg
GTX16443_20180530_IHC-P_18121410_206.jpg
GTX16443_20180530_WB_18121410_714.jpg

Cat No. GTX16443

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Conjugating Enzyme E2 B,E2-17Kda,Hhr6B,Hr6B,Rad6B,Ubc2,Ube2B

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX16443_20180530_IHC-P_1_18121410_576.jpg

GTX16443 IHC-P Image

IHC-P analysis of rat lung tissue using GTX16443 UBE2B antibody.
Dilution : 1:100

GTX16443_20180530_IHC-P_18121410_206.jpg

GTX16443 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX16443 UBE2B antibody.
Dilution : 1:100

GTX16443_20180530_WB_18121410_714.jpg

GTX16443 WB Image

WB analysis of various samples using GTX16443 UBE2B antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane