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UBE2B antibody

Anti-UBE2B antibody used in IHC (Paraffin sections) (IHC-P). GTX16443
Anti-UBE2B antibody used in IHC (Paraffin sections) (IHC-P). GTX16443
Anti-UBE2B antibody used in Western Blot (WB). GTX16443

Cat. No. GTX16443

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all UBE2B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human UBE2B (NP_003328.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 B , E2-17kDa , HHR6B , HR6B , RAD6B , UBC2

Cellular Localization

Cell membrane,Nucleus

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-UBE2B antibody used in IHC (Paraffin sections) (IHC-P). GTX16443

GTX16443 IHC-P Image

IHC-P analysis of rat lung tissue using GTX16443 UBE2B antibody.
Dilution : 1:100

Anti-UBE2B antibody used in IHC (Paraffin sections) (IHC-P). GTX16443

GTX16443 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX16443 UBE2B antibody.
Dilution : 1:100

Anti-UBE2B antibody used in Western Blot (WB). GTX16443

GTX16443 WB Image

WB analysis of various sample lysates using GTX16443 UBE2B antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399