APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-3μg/ml |
| 10μg/ml |
| Assay dependent |
| Assay dependent |
|
Note :
ICC/IF
Strong expression of the protein seen in the cytoplasm of U2OS and MCF7 cells.
|
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89866-PEP).
Product Note
This antibody is expected to recognise all reported isoforms.
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-QETYSKQVTSQEP, from the C Terminus of the protein sequence according to NP_008950.1; NP_861515.1; NP_861516.1; NP_861517.1; NP_861518.1; NP_001268670.1.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 C , UBCH10 , dJ447F3.2
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]
Database
Research Area
DATA IMAGES
|
GTX89866 ICC/IF Image
ICC/IF analysis of PFA fixed MCF7 cells using GTX89866 UBE2C antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
|
|
|
GTX89866 WB Image
WB analysis of HEK293 (A) and HeLa (B) cell lysate using GTX89866 UBE2C antibody, C-term.
Dilution : 2μg/ml
Loading : 35μg protein in RIPA buffer
|
|
|
GTX89866 ICC/IF Image
ICC/IF analysis of PFA fixed U2OS cells using GTX89866 UBE2C antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml
|
|
|
GTX89866 FACS Image
FACS analysis of PFA fixed HeLa cells using GTX89866 UBE2C antibody, C-term.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml
|
REFERENCE
There are currently no references for UBE2C antibody, C-term (GTX89866). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UBE2C antibody, C-term (GTX89866). Be the first to share your experience with this product.
