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United States (US)

UBE2C antibody

Anti-UBE2C antibody used in Immunoprecipitation (IP). GTX33567
Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567
Anti-UBE2C antibody used in Western Blot (WB). GTX33567
Anti-UBE2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33567
Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567

Cat No. GTX33567

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

20 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Conjugating Enzyme E2 C,Ubch10,Dj447F3.2,Ube2C

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES
Anti-UBE2C antibody used in Immunoprecipitation (IP). GTX33567

GTX33567 IP Image

IP analysis of A549 cell lysate using GTX33567 UBE2C antibody.
IP reaction : 3μg antibody / 150μg cell lysate
Dilution : 1:500

Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567

GTX33567 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33567 UBE2C antibody.
Dilution : 1:100

Anti-UBE2C antibody used in Western Blot (WB). GTX33567

GTX33567 WB Image

WB analysis of various samples using GTX33567 UBE2C antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-UBE2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33567

GTX33567 ICC/IF Image

ICC/IF analysis of HeLa cell using GTX33567 UBE2C antibody.
Blue : DAPI

Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567

GTX33567 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33567 UBE2C antibody.
Dilution : 1:100

Package List Price ($)
$ 329