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UBE2C antibody

Anti-UBE2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33567
Anti-UBE2C antibody used in Immunoprecipitation (IP). GTX33567
Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567
Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567
Anti-UBE2C antibody used in Western Blot (WB). GTX33567

Cat No. GTX33567

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP

Reactivity

Human
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:2000
ICC/IF 1:50-1:100
IHC-P 1:50-1:200
IP 1:20-1:50
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human UBE2C (NP_008950.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 C , UBCH10 , dJ447F3.2

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is required for the destruction of mitotic cyclins and for cell cycle progression, and may be involved in cancer progression. Multiple transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene have been defined on chromosomes 4, 14, 15, 18, and 19. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-UBE2C antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33567

GTX33567 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33567 UBE2C antibody.
Blue : DAPI

Anti-UBE2C antibody used in Immunoprecipitation (IP). GTX33567

GTX33567 IP Image

IP analysis of SW480 cells using GTX33567 UBE2C antibody.
IP reaction : 150μg extracts / 3μg antibody antibody
Dilution : 1:500

Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567

GTX33567 IHC-P Image

IHC-P analysis of human gastric cancer tissue using GTX33567 UBE2C antibody.
Dilution : 1:100

Anti-UBE2C antibody used in IHC (Paraffin sections) (IHC-P). GTX33567

GTX33567 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX33567 UBE2C antibody.
Dilution : 1:100

Anti-UBE2C antibody used in Western Blot (WB). GTX33567

GTX33567 WB Image

WB analysis of various sample lysates using GTX33567 UBE2C antibody.
Loading : 25ug per lane
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 329