GeneTex
United States (US)

UBE2D1 antibody

GTX16400_20180530_IHC-P_1_18121410_337.jpg
GTX16400_20180530_IHC-P_18121410_215.jpg
GTX16400_20180530_WB_18121410_811.jpg
GTX16400_20180530_IHC-P_2_18121410_622.jpg

Cat No. GTX16400

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

17 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human UBE2D1 (NP_003329.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Conjugating Enzyme E2 D1,E2(17)Kb1,Sft,Ubc4/5,Ubch5,Ubch5A,Ube2D1

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]

Database

Research Area

DATA IMAGES
GTX16400_20180530_IHC-P_1_18121410_337.jpg

GTX16400 IHC-P Image

IHC-P analysis of rat brain tissue using GTX16400 UBE2D1 antibody.
Dilution : 1:100

GTX16400_20180530_IHC-P_18121410_215.jpg

GTX16400 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX16400 UBE2D1 antibody.
Dilution : 1:100

GTX16400_20180530_WB_18121410_811.jpg

GTX16400 WB Image

WB analysis of various samples using GTX16400 UBE2D1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX16400_20180530_IHC-P_2_18121410_622.jpg

GTX16400 IHC-P Image

IHC-P analysis of rat spleen tissue using GTX16400 UBE2D1 antibody.
Dilution : 1:100

Package List Price ($)
$ 319