APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
293T , human fetal liver , human fetal lung , human UBE2 recombinant protein , Jurkat , MCF-7
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human UBE2D3
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 D3 , E2(17)KB3 , UBC4/5 , UBCH5C
Cellular Localization
Cell membrane,Endosome membrane
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme functions in the ubiquitination of the tumor-suppressor protein p53, which is induced by an E3 ubiquitin-protein ligase. [provided by RefSeq, Jan 2017]
Database
Research Area
DATA IMAGES
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GTX46939 WB Image
WB analysis of human fetal liver tissue using GTX46939 UBE2D3 antibody at 1μg/ml.
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GTX46939 WB Image
WB analysis of 293T cells using GTX46939 UBE2D3 antibody at 1μg/ml.
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GTX46939 WB Image
WB analysis of human UBE2 recombinant protein using GTX46939 UBE2D3 antibody at 1:500.
Sample type:
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein
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GTX46939 WB Image
WB analysis of human fetal lung tissue using GTX46939 UBE2D3 antibody at 1μg/ml.
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GTX46939 WB Image
WB analysis of Jurkat cells using GTX46939 UBE2D3 antibody at 1μg/ml.
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GTX46939 WB Image
WB analysis of MCF-7 cells using GTX46939 UBE2D3 antibody at 1μg/ml.
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GTX46939 WB Image
WB analysis of MCF-7 cells using GTX46939 UBE2D3 antibody at 0.2-1μg/ml.
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REFERENCE
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REVIEW
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