APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.2-2.5 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
HT1080 , human UBE2E3 recombinant protein
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human UBE2E3
Purification
Affinity Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 E3 , UBCH9 , UbcM2
Cellular Localization
Nucleus,Cytoplasm
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]
Database
Research Area
DATA IMAGES
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GTX46934 WB Image
WB analysis of HT1080 cells using GTX46934 UBE2E3 antibody at 0.2-1μg/ml.
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GTX46934 WB Image
WB analysis of human UBE2E3 recombinant protein using GTX46934 UBE2E3 antibody at 1:500.
Lanes:
1: 40ng HIS-UBE2D1 protein
2: 40ng HIS-UBE2D2 protein
3: 40ng HIS-UBE2D3 protein
4: 40ng HIS-UBE2D4 protein
5: 40ng HIS-UBE2E1 protein
6: 40ng HIS-UBE2E2 protein
7: 40ng HIS-UBE2E3 protein
8: 40ng HIS-UBE2K protein
9: 40ng HIS-UBE2L3 protein
10: 40ng HIS-UBE2N protein
11: 40ng HIS-UBE2V1 protein
12: 40ng HIS-UBE2V2 protein
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REFERENCE
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REVIEW
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