APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1/500 - 1/2000 |
| 1/200 - 1/1000 |
| 1/200 - 1/1000 |
| 1/200 - 1/400 |
| 1/10000 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.03% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Purified recombinant fragment of human UBE2I expressed in E. Coli.
Purification
Unpurified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 I , C358B7.1 , P18 , UBC9
Cellular Localization
Nucleus,Cytoplasm
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512](/templates/default/js/lightbox/images/loading.gif "Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512")
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GTX60512 IHC-P Image
IHC-P analysis of bladder cancer tissue using GTX60512 UBE2I antibody [1B10].
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GTX60512 ICC/IF Image
ICC/IF analysis of HepG2 cells using GTX60512 UBE2I antibody [1B10].
Green : UBE2I
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments
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GTX60512 WB Image
WB analysis of human UBE2I (AA: 1-158) recombinant protein using GTX60512 UBE2I antibody [1B10].
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GTX60512 ELISA Image
ELISA analysis of antigen using GTX60512 UBE2I antibody [1B10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng
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GTX60512 FACS Image
FACS analysis of HepG2 cells using GTX60512 UBE2I antibody [1B10].
Green : UBE2I
Red : negative control
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GTX60512 WB Image
WB analysis of HeLa (1), HepG2 (2), and Cos7 (3) cell lysate using GTX60512 UBE2I antibody [1B10].
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GTX60512 IHC-P Image
IHC-P analysis of human prostate tissue using GTX60512 UBE2I antibody [1B10].
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REFERENCE
There are currently no references for UBE2I antibody [1B10] (GTX60512). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UBE2I antibody [1B10] (GTX60512). Be the first to share your experience with this product.
![Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_IHC-P_w_23061123_539.webp "Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512")
![Anti-UBE2I antibody [1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_ICCIF_w_23061123_584.webp "Anti-UBE2I antibody [1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60512")
![Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_WB_1_w_23061123_913.webp "Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512")
![Anti-UBE2I antibody [1B10] used in ELISA (ELISA). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_ELISA_w_23061123_798.webp "Anti-UBE2I antibody [1B10] used in ELISA (ELISA). GTX60512")
![Anti-UBE2I antibody [1B10] used in Flow cytometry (FACS). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_FACS_w_23061123_429.webp "Anti-UBE2I antibody [1B10] used in Flow cytometry (FACS). GTX60512")
![Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_WB_w_23061123_916.webp "Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512")
![Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512](/upload/website/prouct_img/small/GTX60512/GTX60512_20170912_IHC-P_1_w_23061123_127.webp "Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512")
![Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_IHC-P_w_23061123_539.webp "Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512")
![Anti-UBE2I antibody [1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_ICCIF_w_23061123_584.webp "Anti-UBE2I antibody [1B10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60512")
![Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_WB_1_w_23061123_913.webp "Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512")
![Anti-UBE2I antibody [1B10] used in ELISA (ELISA). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_ELISA_w_23061123_798.webp "Anti-UBE2I antibody [1B10] used in ELISA (ELISA). GTX60512")
![Anti-UBE2I antibody [1B10] used in Flow cytometry (FACS). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_FACS_w_23061123_429.webp "Anti-UBE2I antibody [1B10] used in Flow cytometry (FACS). GTX60512")
![Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_WB_w_23061123_916.webp "Anti-UBE2I antibody [1B10] used in Western Blot (WB). GTX60512")
![Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512](/upload/website/prouct_img/normal/GTX60512/GTX60512_20170912_IHC-P_1_w_23061123_127.webp "Anti-UBE2I antibody [1B10] used in IHC (Paraffin sections) (IHC-P). GTX60512")