Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:1000.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2I antibody (GTX129293) diluted at 1:1000.

UBC9 antibody detects UBC9 protein at nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
UBC9 antibody (GTX129293) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min