APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
293T , A431 , HeLa , HepG2 , mouse testis
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.7 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UBE2I. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886244
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 I , C358B7.1 , P18 , UBC9
Cellular Localization
Nucleus
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX130355 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with UBE2I antibody (GTX130355) diluted at 1:1000 and competitor's antibody (SC-10759) diluted by 1:500.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX130355 WB Image
UBC9 antibody detects UBC9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBC9 antibody (GTX130355) diluted by 1:1000.
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GTX130355 IP Image
Immunoprecipitation of UBE2I protein from 293T whole cell extracts using 5 μg of UBE2I antibody (GTX130355).
Western blot analysis was performed using UBE2I antibody (GTX130355).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX130355 IHC-P Image
UBE2I antibody detects UBE2I protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human tonsil.UBE2I stained by UBE2I antibody (GTX130355) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130355 WB Image
UBC9 antibody detects UBC9 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 15 % SDS-PAGE, and the membrane was blotted with UBC9 antibody (GTX130355) diluted by 1:1600.
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REFERENCE
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REVIEW
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