APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
0.2-2.5 ug/ml |
2-10 ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat
PROPERTIES
Form
Liquid
Buffer
PBS, 2% Sucrose
Preservative
0.09% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthetic peptide corresponding to a N-terminal region of Human UBE2J1
Purification
Protein A purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 J1 , CGI-76 , HSPC153 , HSPC205 , HSU93243 , NCUBE-1 , NCUBE1 , UBC6 , UBC6E , Ubc6p
Cellular Localization
Endoplasmic reticulum membrane
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is located in the membrane of the endoplasmic reticulum (ER) and may contribute to quality control ER-associated degradation by the ubiquitin-proteasome system. [provided by RefSeq, Jul 2008]
Database
DATA IMAGES
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GTX46922 IHC-P Image
IHC-P analysis of human kidney tissue using GTX46922 UBE2J1 antibody at 4.0-8.0μg/ml.
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GTX46922 WB Image
WB analysis of Jurkat cells using GTX46922 UBE2J1 antibody at 2.5μg/ml.
Lane A: marker
Lane B: Jurkat cells
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GTX46922 IHC-P Image
IHC-P analysis of human lung tissue using GTX46922 UBE2J1 antibody at 4.0-8.0μg/ml.
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REFERENCE
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REVIEW
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