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UBE2L3 antibody, C-term

Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721
Anti-UBE2L3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89721
Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721
Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721
Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721

Cat. No. GTX89721

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat, Pig
Package
100 μg ($399)
See all UBE2L3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
ICC/IF 5μg/ml

Note :

ICC/IF
Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression.
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89721-PEP).

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS pH7.3, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence according to NP_003338.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4

Cellular Localization

Nucleus , Cytoplasm

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721

GTX89721 WB Image

WB analysis of HeLa lysate using GTX89721 UBE2L3 antibody, C-term.
Dilution : 0.5μg/ml
Loading : 35μg protein in RIPA buffer

Anti-UBE2L3 antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX89721

GTX89721 ICC/IF Image

ICC/IF analysis of MCF7 (A) and HeLa (B) cells using GTX89721 UBE2L3 antibody, C-term.
Dilution : 5μg/ml

Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721

GTX89721 WB Image

WB analysis of HEK293 (A), HepG2 (B) and Jurkat (C) lysates using GTX89721 UBE2L3 antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721

GTX89721 WB Image

WB analysis of human (A), mouse (B) and rat (C) skeletal muscle lysates using GTX89721 UBE2L3 antibody, C-term.
Dilution : 0.03μg/ml
Loading : 35μg protein in RIPA buffer

Anti-UBE2L3 antibody, C-term used in Western Blot (WB). GTX89721

GTX89721 WB Image

WB analysis of human (A), mouse (B), rat (C) and pig (D) heart lysate using GTX89721 UBE2L3 antibody, C-term.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399