Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed HeLa, using UBE2L3(GTX114870) antibody at 1:200 dilution.

Sample (30 ug of whole cell lysate)
A: JurKat
B: Hela
12% SDS PAGE
GTX114870 diluted at 1:1000

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000.

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using UBE2L3 antibody [N1C3] (GTX114870) at 1:300 dilution.

Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000.