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UBE2L3 antibody [N1C3]

Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870
Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870
Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870
Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870

Cat No. GTX114870

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

293T , HeLa , JurKat , mouse testis , UBE2L3 shRNA-transfected 293T

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2L3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10624592

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870

GTX114870 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UBE2L3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114870

GTX114870 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UBE2L3(GTX114870) antibody at 1:200 dilution.

Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870

GTX114870 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody [N1C3] (GTX114870) diluted at 1:1000.

Anti-UBE2L3 antibody [N1C3] used in Western Blot (WB). GTX114870

GTX114870 WB Image

Sample (30 ug of whole cell lysate)
A: JurKat
B: Hela
12% SDS PAGE
GTX114870 diluted at 1:1000

REFERENCE

There are currently no references for UBE2L3 antibody [N1C3] (GTX114870). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for UBE2L3 antibody [N1C3] (GTX114870). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169