APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , GL261 , C8D30 , HeLa , HepG2 , mouse testis
Predict Reactivity
Rat, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2L3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1241426
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4
Background
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX104717 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(GTX104717) antibody at 1:200 dilution.
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GTX104717 WB Image
UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (GTX104717) dilution: 1:1000
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GTX104717 IHC-P Image
Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(GTX104717) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX104717 WB Image
Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.
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GTX104717 WB Image
Whole zebrafish extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.
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GTX104717 IP Image
Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 μg of UBE2L3 antibody (GTX104717).
Western blot analysis was performed using UBE2L3 antibody (GTX104717) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX104717 WB Image
Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX104717 diluted at 1:1000
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REFERENCE
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