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UBE2L3 antibody

Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717
Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717
Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717
Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104717
Anti-UBE2L3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104717
Anti-UBE2L3 antibody used in Immunoprecipitation (IP). GTX104717
Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717

Cat No. GTX104717

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Zebrafish
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa , HepG2 , Neuro2A , GL261 , C8D30 , mouse testis , whole zebrafish

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2L3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717

GTX104717 WB Image

Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717

GTX104717 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717

GTX104717 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX104717 diluted at 1:1000

Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX104717

GTX104717 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(GTX104717) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UBE2L3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104717

GTX104717 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(GTX104717) antibody at 1:200 dilution.

Anti-UBE2L3 antibody used in Immunoprecipitation (IP). GTX104717

GTX104717 IP Image

Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 μg of UBE2L3 antibody (GTX104717).
Western blot analysis was performed using UBE2L3 antibody (GTX104717).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-UBE2L3 antibody used in Western Blot (WB). GTX104717

GTX104717 WB Image

UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (GTX104717) dilution: 1:1000

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169