GeneTex
United States (US)

UBE2L3 antibody

GTX104717_39652_20160818_WB_Z_19011506_577.jpg
GTX104717_39652_20160818_WB_M_testis_19011506_957.jpg
GTX104717_WB_19011506_174.jpg
GTX104717_39652_IHC_19011506_139.jpg
GTX104717_IFA_19011506_609.jpg
GTX104717_39652_IP_19011506_864.jpg
GTX104717_39652_WB_M_19011506_159.jpg

Cat No. GTX104717

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IP
Reactivity Human, Mouse, Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

HeLa , HepG2 , Neuro2A , GL261 , C8D30 , mouse testis , whole zebrafish

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2L3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

E2-F1 antibody, L-UBC antibody, UBCH7 antibody, UbcM4 antibody, UBE2L3 antibody, ubiquitin-conjugating enzyme E2 L3 antibody, ubiquitin-protein ligase L3 antibody, ubiquitin carrier protein L3 antibody, ubiquitin-conjugating enzyme E2-F1 antibody, ubiquitin-conjugating enzyme UBCH7 antibody, ubiquitin-conjugating enzyme E2L 3 antibody

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX104717_39652_20160818_WB_Z_19011506_577.jpg

GTX104717 WB Image

Whole zebrafish extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

GTX104717_39652_20160818_WB_M_testis_19011506_957.jpg

GTX104717 WB Image

Mouse tissue extract (50 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with UBE2L3 antibody (GTX104717) diluted at 1:1000.

GTX104717_WB_19011506_174.jpg

GTX104717 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX104717 diluted at 1:1000

GTX104717_39652_IHC_19011506_139.jpg

GTX104717 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using UBE2L3(GTX104717) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104717_IFA_19011506_609.jpg

GTX104717 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UBE2L3(GTX104717) antibody at 1:200 dilution.

GTX104717_39652_IP_19011506_864.jpg

GTX104717 IP Image

Immunoprecipitation of UBE2L3 protein from HeLa whole cell extracts using 5 μg of UBE2L3 antibody (GTX104717).
Western blot analysis was performed using UBE2L3 antibody (GTX104717).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX104717_39652_WB_M_19011506_159.jpg

GTX104717 WB Image

UBE2L3 antibody detects UBE2L3 protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 ug GL261 whole cell lysate/extract
C. 30 ug C8D30 whole cell lysate/extract
15 % SDS-PAGE
UBE2L3 antibody (GTX104717) dilution: 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169