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UBE2L3 antibody

Anti-UBE2L3 antibody used in Western Blot (WB). GTX33568
Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568
Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568
Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568
Anti-UBE2L3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33568

Cat. No. GTX33568

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all UBE2L3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 73-212 of human UBE2L3 (NP_001243284.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 L3 , E2-F1 , L-UBC , UBCH7 , UbcM4

Cellular Localization

Nucleus,Cytoplasm

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-UBE2L3 antibody used in Western Blot (WB). GTX33568

GTX33568 WB Image

WB analysis of various sample lysates using GTX33568 UBE2L3 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568

GTX33568 IHC-P Image

IHC-P analysis of rat heart tissue using GTX33568 UBE2L3 antibody.
Dilution : 1:100

Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568

GTX33568 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX33568 UBE2L3 antibody.
Dilution : 1:100

Anti-UBE2L3 antibody used in IHC (Paraffin sections) (IHC-P). GTX33568

GTX33568 IHC-P Image

IHC-P analysis of human stomach tissue using GTX33568 UBE2L3 antibody.
Dilution : 1:100

Anti-UBE2L3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33568

GTX33568 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX33568 UBE2L3 antibody.
Blue : DAPI
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399