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UBE2L6 antibody [k1H3]

Anti-UBE2L6 antibody [k1H3] used in IHC (Paraffin sections) (IHC-P). GTX53796
Anti-UBE2L6 antibody [k1H3] used in Western Blot (WB). GTX53796

Cat No. GTX53796

Host

Mouse

Clonality

Monoclonal

Clone Name

k1H3

Isotype

IgG2b

Application

WB, IHC-P, ELISA

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:3,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:2,000 and Immunohistochemistry is 1:50.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human UBE2L6 (1-152aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Ubiquitin Conjugating Enzyme E2 L6 , Rig-B , Ubch8 , Ube2L6

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]

Database

Research Area

DATA IMAGES

Anti-UBE2L6 antibody [k1H3] used in IHC (Paraffin sections) (IHC-P). GTX53796

GTX53796 IHC-P Image

IHC-P analysis of human breast lobule tissue using UBE2L6 antibody at a dilution of 1:50.

Anti-UBE2L6 antibody [k1H3] used in Western Blot (WB). GTX53796

GTX53796 WB Image

WB analysis of MCF7 lysate (20ug) using UBE2L6 antibody at a dilution of 1:2,000.

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf