GeneTex
United States (US)

UBE2L6 antibody [k1H3]

GTX53796_WB_18121410_130.jpg
GTX53796_IHC_18121410_857.jpg

Cat No. GTX53796

Host Mouse
Clonality Monoclonal
Clone Name k1H3
Isotype IgG2b
Application WB, IHC-P, ELISA
Reactivity Human
APPLICATION

Application Note

The antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:1,000~1:3,000 and immunohistochemistry analysis is 1:50~100. Recommended starting dilution for Western blot is 1:2,000 and Immunohistochemistry is 1:50.

Calculated MW

18 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human UBE2L6 (1-152aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

UBCH8 Antibody , ubiquitin-conjugating enzyme E2L 6 Antibody , RIG-B Antibody

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is highly similar in primary structure to the enzyme encoded by the UBE2L3 gene. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2011]

Database

DATA IMAGES
GTX53796_WB_18121410_130.jpg

GTX53796 WB Image

WB analysis of MCF7 lysate (20ug) using UBE2L6 antibody at a dilution of 1:2,000.

GTX53796_IHC_18121410_857.jpg

GTX53796 IHC-P Image

IHC-P analysis of human breast lobule tissue using UBE2L6 antibody at a dilution of 1:50.

Package List Price ($)
$ 319