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UBE2N antibody

Anti-UBE2N antibody used in Western Blot (WB). GTX113290
Anti-UBE2N antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113290
Anti-UBE2N antibody used in Western Blot (WB). GTX113290

Cat. No. GTX113290

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($419),
25 μl ($169)
See all UBE2N products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Positive Control

Mouse brain , A431 , HepG2

Predict Reactivity

Rat, Sheep, Bovine, Dog, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE2N. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038198

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 N , HEL-S-71 , UBC13 , UBCHBEN; UBC13 , UbcH-ben , UbcH13

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Studies in mouse suggest that this protein plays a role in DNA postreplication repair. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE2N antibody used in Western Blot (WB). GTX113290

GTX113290 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113290 diluted at 1:1000

Anti-UBE2N antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113290

GTX113290 ICC/IF Image

UBE2N antibody detects UBE2N protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE2N protein stained by UBE2N antibody (GTX113290) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-UBE2N antibody used in Western Blot (WB). GTX113290

GTX113290 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: Hep G2 (GTX27900)
12% SDS PAGE
GTX113290 diluted at 1:1000

REFERENCE

Application Reference

S He et al. Transl Psychiatry 2023; 13 (1) : 362 Does decreased autophagy and dysregulation of LC3A in astrocytes play a role in major depressive disorder?
Application : WB
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 419
$ 169