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UBE2O antibody

Anti-UBE2O antibody used in Immunoprecipitation (IP). GTX35205
Anti-UBE2O antibody used in Western Blot (WB). GTX35205
Anti-UBE2O antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35205
Anti-UBE2O antibody used in Western Blot (WB). GTX35205

Cat. No. GTX35205

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000 - 1:4000
ICC/IF 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1073-1292 of human UBE2O (NP_071349.3).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 O , E2-230K

Cellular Localization

Cytoplasm,Nucleus

Database

Research Area

DATA IMAGES

Anti-UBE2O antibody used in Immunoprecipitation (IP). GTX35205

GTX35205 IP Image

IP analysis of A431 cell lysate using GTX35205 UBE2O antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-UBE2O antibody used in Western Blot (WB). GTX35205

GTX35205 WB Image

WB analysis of various sample lysates using GTX35205 UBE2O antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-UBE2O antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35205

GTX35205 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35205 UBE2O antibody.
Blue : DAPI
Dilution : 1:100

Anti-UBE2O antibody used in Western Blot (WB). GTX35205

GTX35205 WB Image

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35205 UBE2O antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399