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UBE2T antibody [2F5]

Overexpression
Anti-UBE2T antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83452
Anti-UBE2T antibody [2F5] used in Western Blot (WB). GTX83452
Anti-UBE2T antibody [2F5] used in Western Blot (WB). GTX83452

Cat. No. GTX83452

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2F5

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Dog
Package
100 μl ($519)
See all UBE2T products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:100
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.33 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human UBE2T (NP_054895) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin conjugating enzyme E2 T , FANCT , HSPC150 , PIG50

Cellular Localization

Nucleus

Background

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

Database

Research Area

DATA IMAGES

Anti-UBE2T antibody [2F5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83452

GTX83452 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with UBE2T plasmid using GTX83452 UBE2T antibody [2F5].

Anti-UBE2T antibody [2F5] used in Western Blot (WB). GTX83452

GTX83452 WB Image

Overexpression

WB analysis of HEK293T cells transfected with UBE2T plasmid (Right) or empty vector (Left) for 48 hrs using GTX83452 UBE2T antibody [2F5].
Loading : 5 ug per lane

Anti-UBE2T antibody [2F5] used in Western Blot (WB). GTX83452

GTX83452 WB Image

WB analysis of various cell lines using GTX83452 UBE2T antibody [2F5].
Loading : 35 ug per lane
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519