GeneTex
United States (US)

UBE3A antibody

GTX101092_41087_20180503_ICC_IF_R_19011506_736.jpg
GTX101092_41087_IHC_M_19011506_331.jpg
GTX101092_41087_IHC_19011506_750.jpg

Cat No. GTX101092

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Predict Reactivity

Bovine, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBE3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ANCR Antibody, HPVE6A Antibody, EPVE6AP Antibody, ubiquitin protein ligase E3A Antibody, E6-AP Antibody, AS Antibody

Cellular Localization

Nucleus

Background

This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101092_41087_20180503_ICC_IF_R_19011506_736.jpg

GTX101092 ICC/IF Image

UBE3A antibody detects UBE3A protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UBE3A stained by UBE3A antibody (GTX101092) diluted at 1:500.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX101092_41087_IHC_M_19011506_331.jpg

GTX101092 IHC-P Image

UBE3A antibody detects UBE3A protein at cytosol on mouse middle brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse middle brain.
UBE3A antibody (GTX101092) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101092_41087_IHC_19011506_750.jpg

GTX101092 IHC-P Image

UBE3A antibody detects UBE3A protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
UBE3A antibody (GTX101092) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169