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UBE4B antibody [C2C3], C-term

Anti-UBE4B antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109385
Anti-UBE4B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109385
Anti-UBE4B antibody [C2C3], C-term used in Western Blot (WB). GTX109385

Cat. No. GTX109385

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UFD2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

146 kDa. ( Note )

Positive Control

HepG2

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952489

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitination factor E4B , E4 , HDNB1 , UBOX3 , UFD2 , UFD2A

Cellular Localization

Cytoplasm

Background

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UBE4B antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109385

GTX109385 IHC-P Image

Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using UBE4B(GTX109385) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UBE4B antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109385

GTX109385 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using UBE4B(GTX109385) antibody at 1:500 dilution.

Anti-UBE4B antibody [C2C3], C-term used in Western Blot (WB). GTX109385

GTX109385 WB Image

Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX109385 diluted at 1:10000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169