*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji
Mouse, Rat, Zebrafish, Bovine(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
1 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the C-terminus region of human UBE4B. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ubiquitination factor E4B , E4 , HDNB1 , UBOX3 , UFD2 , UFD2A
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes an additional conjugation factor, E4, which is involved in multiubiquitin chain assembly. This gene is also the strongest candidate in the neuroblastoma tumor suppressor genes. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
GTX109385 ICC/IF Image
Immunofluorescence analysis of methanol-fixed Hep3B, using UBE4B(GTX109385) antibody at 1:500 dilution.
GTX109385 WB Image
Sample (30 ug of whole cell lysate)
A: Hep G2 (GTX27900)
5% SDS PAGE
GTX109385 diluted at 1:10000
GTX109385 IHC-P Image
Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using UBE4B(GTX109385) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min