GeneTex
United States (US)

UBR4 antibody, C-term

GTX88365_1006_IHC-P_18121411_528.jpg

Cat No. GTX88365

Host Goat
Clonality Polyclonal
Isotype IgG
Application IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
IHC-P 5-10μg/ml

Note :

IHC-P
Spleen shows various types of subnuclear staining in select splenocytes and additional membrane plus cytoplasm staining elsewhere.

Not tested in other applications.

Calculated MW

574 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88365-PEP).

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-NTTRATSREEKNLQ, from the C Terminus of the protein sequence according to NP_065816.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Ubiquitin Protein Ligase E3 Component N-Recognin 4,Rbaf600,Zubr1,P600,Ubr4

Cellular Localization

Concentrates at the leading edge of membrane structures involved in actin motility.

Background

The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein. [provided by RefSeq, Aug 2010]

Database

DATA IMAGES
GTX88365_1006_IHC-P_18121411_528.jpg

GTX88365 IHC-P Image

IHC-P analysis of human spleen using GTX88365 UBR4 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 5µg/ml

Package List Price ($)
$ 299