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UBTF (phospho Ser484) antibody

Anti-UBTF (phospho Ser484) antibody used in IHC (Paraffin sections) (IHC-P). GTX55450
Anti-UBTF (phospho Ser484) antibody used in Western Blot (WB). GTX55450

Cat. No. GTX55450

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all UBTF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide sequence around phosphorylation site of serine 484 (P-E-S(p)-P-K) derived from human UBTF.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

upstream binding transcription factor , CONDBA , NOR-90 , UBF , UBF-1 , UBF1 , UBF2

Cellular Localization

Nucleolus

Background

This gene encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-UBTF (phospho Ser484) antibody used in IHC (Paraffin sections) (IHC-P). GTX55450

GTX55450 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX55450 UBTF (phospho Ser484) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

Anti-UBTF (phospho Ser484) antibody used in Western Blot (WB). GTX55450

GTX55450 WB Image

WB analysis of extracts from HeLa cells treated with calyculinA (50ng/ml 30mins) using GTX55450 UBTF (phospho Ser484) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399