GeneTex

  • Country / Location Selection

United States (US)

UBXN1 antibody

Anti-UBXN1 antibody used in Western Blot (WB). GTX121127
Anti-UBXN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121127
Anti-UBXN1 antibody used in Western Blot (WB). GTX121127
Anti-UBXN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121127

Cat. No. GTX121127

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UBXN1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

293T , A431 , HeLa , NIH-3T3 , JC , BCL-1

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UBXN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164988

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UBX domain protein 1 , 2B28 , SAKS1 , UBXD10

Cellular Localization

Cytoplasm

Background

Ubiquitin-binding protein that interacts with the BRCA1-BARD1 heterodimer, and regulates its activity. Specifically binds 'Lys-6'-linked polyubiquitin chains. Interaction with autoubiquitinated BRCA1, leads to inhibit the E3 ubiquitin-protein ligase activity of the BRCA1-BARD1 heterodimer. Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulun that are retrotranslocated in the cytosol.

Database

Research Area

DATA IMAGES

Anti-UBXN1 antibody used in Western Blot (WB). GTX121127

GTX121127 WB Image

Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX121127 diluted at 1:3000

Anti-UBXN1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121127

GTX121127 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UBXN1(GTX121127) antibody at 1:500 dilution.

Anti-UBXN1 antibody used in Western Blot (WB). GTX121127

GTX121127 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
10% SDS PAGE
GTX121127 diluted at 1:1000

Anti-UBXN1 antibody used in IHC (Paraffin sections) (IHC-P). GTX121127

GTX121127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UBXN1(GTX121127) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for UBXN1 antibody (GTX121127). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for UBXN1 antibody (GTX121127). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169