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UCHL5 antibody

Anti-UCHL5 antibody used in Immunoprecipitation (IP). GTX131407
Anti-UCHL5 antibody used in Western Blot (WB). GTX131407

Cat No. GTX131407

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.48 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human UCHL5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ubiquitin C-terminal hydrolase L5,CGI-70,INO80R,UCH-L5,UCH37

Background

Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome.

Database

DATA IMAGES
Anti-UCHL5 antibody used in Immunoprecipitation (IP). GTX131407

GTX131407 IP Image

Immunoprecipitation of UCHL5 protein from 293T whole cell extracts using 5 μg of UCHL5 antibody (GTX131407) .
Western blot analysis was performed using UCHL5 antibody (GTX131407).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-UCHL5 antibody used in Western Blot (WB). GTX131407

GTX131407 WB Image

UCHL5 antibody detects UCHL5 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with UCHL5 antibody (GTX131407) diluted at 1:500.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169