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UCK1 antibody [5E9]

Overexpression
Anti-UCK1 antibody [5E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83441
Anti-UCK1 antibody [5E9] used in Western Blot (WB). GTX83441
Anti-UCK1 antibody [5E9] used in Western Blot (WB). GTX83441
Anti-UCK1 antibody [5E9] used in Flow cytometry (FACS). GTX83441

Cat. No. GTX83441

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E9

Isotype

 IgG1

Application

 WB, ICC/IF, FACS

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all UCK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
FACS 1:100
Not tested in other applications.

Calculated MW

31 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human UCK1 (NP_113620) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uridine-cytidine kinase 1 , URK1

Background

Phosphorylates uridine and cytidine to uridine monophosphate and cytidine monophosphate. Does not phosphorylate deoxyribonucleosides or purine ribonucleosides. Can use ATP or GTP as a phosphate donor. Can also phosphorylate cytidine and uridine nucleoside analogs such as 6-azauridine, 5-fluorouridine, 4-thiouridine, 5-bromouridine, N(4)-acetylcytidine, N(4)-benzoylcytidine, 5-fluorocytidine, 2-thiocytidine, 5-methylcytidine, and N(4)-anisoylcytidine.

Database

DATA IMAGES

Anti-UCK1 antibody [5E9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83441

GTX83441 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with UCK1 plasmid using GTX83441 UCK1 antibody [5E9].

Anti-UCK1 antibody [5E9] used in Western Blot (WB). GTX83441

GTX83441 WB Image

WB analysis of various cell lines using GTX83441 UCK1 antibody [5E9].
Loading : 10 ug per lane
Dilution : 1:200

Anti-UCK1 antibody [5E9] used in Western Blot (WB). GTX83441

GTX83441 WB Image

Overexpression

WB analysis of HEK293T cells transfected with UCK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83441 UCK1 antibody [5E9].
Loading : 5 ug per lane

Anti-UCK1 antibody [5E9] used in Flow cytometry (FACS). GTX83441

GTX83441 FACS Image

FACS analysis of HEK293T cells transfected with either UCK1 plasmid(Red) or empty vector control plasmid(Blue) using GTX83441 UCK1 antibody [5E9].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519