GeneTex

  • Country / Location Selection

United States (US)

UCP1 antibody [GT1345]

Anti-UCP1 antibody [GT1345] used in Western Blot (WB). GTX632186
Anti-UCP1 antibody [GT1345] used in IHC (Paraffin sections) (IHC-P). GTX632186

Cat. No. GTX632186

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1345

Isotype

 IgG2a

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all UCP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Predict Reactivity

Human, Rat, Rabbit, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888293

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uncoupling protein 1 , SLC25A7 , UCP

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UCP1 antibody [GT1345] used in Western Blot (WB). GTX632186

GTX632186 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody [GT1345] (GTX632186) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-UCP1 antibody [GT1345] used in IHC (Paraffin sections) (IHC-P). GTX632186

GTX632186 IHC-P Image

UCP1 antibody [GT1345] detects UCP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
UCP1 stained by UCP1 antibody [GT1345] (GTX632186) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Corsetti G et al. Nutrients 2022; 14 (14) Essential Amino Acids-Rich Diet Decreased Adipose Tissue Storage in Adult Mice: A Preliminary Histopathological Study.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

REVIEW

There are currently no reviews for UCP1 antibody [GT1345] (GTX632186). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations