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UCP1 antibody [GT3111]

Anti-UCP1 antibody [GT3111] used in Western Blot (WB). GTX632185
Anti-UCP1 antibody [GT3111] used in IHC (Paraffin sections) (IHC-P). GTX632185

Cat. No. GTX632185

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT3111

Isotype

 IgG1

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all UCP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

mouse brown adipose

Predict Reactivity

Human, Rat, Rabbit, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888292

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uncoupling protein 1 , SLC25A7 , UCP

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UCP1 antibody [GT3111] used in Western Blot (WB). GTX632185

GTX632185 WB Image

UCP1 antibody detects UCP1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX632185) at a dilution of 1:1000.

Anti-UCP1 antibody [GT3111] used in IHC (Paraffin sections) (IHC-P). GTX632185

GTX632185 IHC-P Image

UCP1 antibody [GT3111] detects UCP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
UCP1 stained by UCP1 antibody [GT3111] (GTX632185) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Song G et al. J Agric Food Chem 2020; 68 (24) : 6715-6725 Fruit of Hovenia dulcis Thunb. Induces Nonshivering Thermogenesis through Mitochondrial Biogenesis and Activation by SIRT1 in High-Fat Diet-Fed Obese Mice and Primary Cultured Brown Adipocytes.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations