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UCP1 antibody

Anti-UCP1 antibody used in Western Blot (WB). GTX112784
Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784
Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

Cat No. GTX112784

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

mouse brown adipose

Predict Reactivity

Rat, Rabbit, Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UCP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10623171

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

uncoupling protein 1 , SLC25A7 , UCP

Cellular Localization

Mitochondrion inner membrane; Multi-pass membrane protein

Background

Mitochondrial uncoupling proteins (UCP) are members of the family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed only in brown adipose tissue, a specialized tissue which functions to produce heat. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UCP1 antibody used in Western Blot (WB). GTX112784

GTX112784 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with UCP1 antibody (GTX112784) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

GTX112784 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UCP1(GTX112784) antibody at 1:250 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UCP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112784

GTX112784 IHC-P Image

UCP1 antibody detects UCP1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
UCP1 stained by UCP1 antibody (GTX112784) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

UCP1 antibody Cat No. GTX112784

Rating ( Average 5 based on 4 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2019. PMID: 30959933
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Nutrients in 2019. PMID: 30959933
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal PLoS Biol in 2019. PMID: 30653498
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : brown fat
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Brown fat lysate from mouse housed in room temperature, Lane 2: after 4 oC cold exposure
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 379
$ 169