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UCP2 antibody, Internal

Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094
Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094
Anti-UCP2 antibody, Internal used in Western Blot (WB). GTX89094
Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094

Cat. No. GTX89094

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all UCP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

33 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89094-PEP).

Predict Reactivity

Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-DSVKQFYTKGSEH, from the internal region of the protein sequence according to NP_003346.2.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uncoupling protein 2 , BMIQ4 , SLC25A8 , UCPH

Background

Mitochondrial uncoupling proteins (UCP) are members of the larger family of mitochondrial anion carrier proteins (MACP). UCPs separate oxidative phosphorylation from ATP synthesis with energy dissipated as heat, also referred to as the mitochondrial proton leak. UCPs facilitate the transfer of anions from the inner to the outer mitochondrial membrane and the return transfer of protons from the outer to the inner mitochondrial membrane. They also reduce the mitochondrial membrane potential in mammalian cells. Tissue specificity occurs for the different UCPs and the exact methods of how UCPs transfer H+/OH- are not known. UCPs contain the three homologous protein domains of MACPs. This gene is expressed in many tissues, with the greatest expression in skeletal muscle. It is thought to play a role in nonshivering thermogenesis, obesity and diabetes. Chromosomal order is 5'-UCP3-UCP2-3'. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094

GTX89094 IHC-P Image

IHC-P analysis of human skeletal muscle using GTX89094 UCP2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094

GTX89094 IHC-P Image

IHC-P analysis of human adipocytes using GTX89094 UCP2 antibody, Internal.
Antigen retrieval : citrate buffer Ph 6
Dilution : 3.75μg/ml

Anti-UCP2 antibody, Internal used in Western Blot (WB). GTX89094

GTX89094 WB Image

WB analysis of rat adipose lysate using GTX89094 UCP2 antibody, Internal.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-UCP2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX89094

GTX89094 IHC-P Image

IHC-P analysis of human lung using GTX89094 UCP2 antibody, Internal.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399