GeneTex
United States (US)

UEA I Lectin

Cat No. GTX01510

Application Conjugation, Puri
PRODUCT

Product Description

Ulex europaeus lectin against fucose blood group H
APPLICATION

Application Note

Conjugation to Sepharose 4B (solid phase columns) to purify blood group glycoproteins. For conjugation azide should be removed. Dilute in buffer containing 0.1 mM calcium chloride. The optimum dilution should be determined by the individual lab.

Specificity/Sensitivity

Fucose blood group H
PROPERTIES

Form

Liquid

Buffer

10 mM phosphate, 150 mM NaCl, pH 7.6, 0.1 mM Calcium chloride and 0.05% sodium azide

Storage

Store at 2-8ºC.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

UAE, Ulex europaeus, UAE I Lectin, Fucose lectin, UAE I, Ulex europaeus lectin, UAE Lectin

Background

The anti-H (O) hemagglutinating activity of Ulex europaeus has been used widely to confirm blood group O activity. UEA I binds to many glycoproteins and glycolipids containing α –linked fucose residue. The purified lectin appears to be a dimer of two distinct polypeptide chains associated by noncovalent forces. This lectin does not react with Lea active blood group substance. The native protein has pI=6.0-6.1 and exhibits a Molecular weight 60,000-68,000 during gel filtration on Sephadex column. This lectin will hemagglutinate fresh blood group O cells at 2-5μg/ml.Inhibiting/Eluting sugars: 50-100 mM L-fucoseCarbohydrate-Binding Specificity of UEA I: L-Fuα1,2Galβ1,4GlcNAcβ1,6R
Package List Price ($)
$ 99