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UFD1L antibody

Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579
Anti-UFD1L antibody used in Western Blot (WB). GTX109579
Anti-UFD1L antibody used in Western Blot (WB). GTX109579
Anti-UFD1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109579
Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579
Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579

Cat. No. GTX109579

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UFD1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431 , H1299 , mouse lung

Predict Reactivity

Rat, Zebrafish, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.02 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human UFD1L Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952503

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin recognition factor in ER associated degradation 1 , UFD1L

Cellular Localization

Nucleus , Cytoplasm , cytosol

Background

The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, that is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579

GTX109579 IHC-P Image

UFD1L antibody detects UFD1L protein at cytoplasm and nucleus in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UFD1L antibody (GTX109579) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UFD1L antibody used in Western Blot (WB). GTX109579

GTX109579 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
10% SDS PAGEGTX109579 diluted at 1:10000

Anti-UFD1L antibody used in Western Blot (WB). GTX109579

GTX109579 WB Image

UFD1L antibody detects UFD1L protein by Western blot analysis.
A. 50 μg mouse lung lysate/extract
12 % SDS-PAGE
UFD1L antibody (GTX109579) dilution: 1:1000

Anti-UFD1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109579

GTX109579 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using UFD1L(GTX109579) antibody at 1:200 dilution.

Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579

GTX109579 IHC-P Image

UFD1L antibody detects UFD1L protein at cytoplasm and nucleus in mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
UFD1L antibody (GTX109579) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX109579

GTX109579 IHC-P Image

Immunohistochemical analysis of paraffin-embedded H441 xenograft, using UFD1L(GTX109579) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Tsalikis J et al. Mol Cell Biol 2019; 39 (10) Isoginkgetin, a Natural Biflavonoid Proteasome Inhibitor, Sensitizes Cancer Cells to Apoptosis via Disruption of Lysosomal Homeostasis and Impaired Protein Clearance.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169