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UFD1L antibody

Anti-UFD1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118138
Anti-UFD1L antibody used in Western Blot (WB). GTX118138
Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX118138

Cat. No. GTX118138

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UFD1L products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UFD1L. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10616685

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin recognition factor in ER associated degradation 1 , UFD1L

Cellular Localization

Nucleus , Cytoplasm , cytosol

Background

The protein encoded by this gene forms a complex with two other proteins, NPL4 and VCP, that is necessary for the degradation of ubiquitinated proteins. In addition, this complex controls the disassembly of the mitotic spindle and the formation of a closed nuclear envelope after mitosis. Mutations in this gene have been associated with Catch 22 syndrome as well as cardiac and craniofacial defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UFD1L antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX118138

GTX118138 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using UFD1L(GTX118138) antibody at 1:200 dilution.

Anti-UFD1L antibody used in Western Blot (WB). GTX118138

GTX118138 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX118138 diluted at 1:1000

Anti-UFD1L antibody used in IHC (Paraffin sections) (IHC-P). GTX118138

GTX118138 IHC-P Image

UFD1L antibody detects UFD1L protein at cytosol on H1299 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded H1299 xenograft.
UFD1L antibody (GTX118138) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169