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UGCGL2 antibody [N3C2], Internal

Anti-UGCGL2 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103837
Anti-UGCGL2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103837
Anti-UGCGL2 antibody [N3C2], Internal used in Western Blot (WB). GTX103837

Cat. No. GTX103837

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all UGCGL2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

175 kDa. ( Note )

Positive Control

HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UGCGL2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UDP-glucose glycoprotein glucosyltransferase 2 , HUGT2 , UGCGL2 , UGT2

Cellular Localization

Endoplasmic reticulum lumen , Endoplasmic reticulum-Golgi intermediate compartment

Background

UDP-glucose:glycoprotein glucosyltransferase (UGT) is a soluble protein of the endoplasmic reticulum (ER) that selectively reglucosylates unfolded glycoproteins, thus providing quality control for protein transport out of the ER.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-UGCGL2 antibody [N3C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103837

GTX103837 ICC/IF Image

UGCGL2 antibody [N3C2], Internal detects UGCGL2 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: UGCGL2 protein stained by UGCGL2 antibody [N3C2], Internal (GTX103837) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-UGCGL2 antibody [N3C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103837

GTX103837 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using UGCGL2(GTX103837) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UGCGL2 antibody [N3C2], Internal used in Western Blot (WB). GTX103837

GTX103837 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
5% SDS PAGE
GTX103837 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169