GeneTex
United States (US)

UGDH antibody

Overexpression
GTX104993_40849_20180720_WB_B_19011506_778.jpg
GTX104993_40849_WB_19011506_489.jpg
GTX104993_40849_IHC_19011506_642.jpg
GTX104993_40849_WB_M_Liver_19011506_862.jpg
GTX104993_40849_WB_R_liver_19011506_716.jpg

Cat No. GTX104993

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

Positive Control

HepG2 , mouse liver , rat liver , UGDH-transfected 293T

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GDH antibody, UDP-GlcDH antibody, UDPGDH antibody, UGD antibody, UGDH antibody, UDP-glucose 6-dehydrogenase antibody, UDP-Glc dehydrogenase antibody, UDP-glucose dehydrogenase antibody, uridine diphospho-glucose dehydrogenase antibody

Background

The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. [provided by RefSeq]

Database

DATA IMAGES
GTX104993_40849_20180720_WB_B_19011506_778.jpg

GTX104993 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with UGDH antibody (GTX104993) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX104993_40849_WB_19011506_489.jpg

GTX104993 WB Image

UGDH antibody detects UGDH protein by Western blot analysis.
A. 30 µg HepG2 whole cell lysate/extract
7.5 % SDS-PAGE
UGDH antibody (GTX104993) dilution: 1:10000

GTX104993_40849_IHC_19011506_642.jpg

GTX104993 IHC-P Image

UGDH antibody detects UGDH protein at cytosol on human hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma tissue.
UGDH antibody (GTX104993) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX104993_40849_WB_M_Liver_19011506_862.jpg

GTX104993 WB Image

Sample (50 ug of whole cell lysate)
A: mouse liver
7.5% SDS PAGE
GTX104993 diluted at 1:10000

GTX104993_40849_WB_R_liver_19011506_716.jpg

GTX104993 WB Image

UGDH antibody detects UGDH protein by Western blot analysis.
A. 50 µg Rat liver lysate/extract
7.5 % SDS-PAGE
UGDH antibody (GTX104993) dilution: 1:2000

REVIEW

UGDH antibody Cat No. GTX104993

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : TOV-21G cells
Amount used : 60 μg
Blocking : 5 % milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169