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UGP2 antibody [N3C3-2]

KO/KD
Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967
Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967
Anti-UGP2 antibody [N3C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107967
Anti-UGP2 antibody [N3C3-2] used in Western Blot (WB). GTX107967
Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967
Anti-UGP2 antibody [N3C3-2] used in Western Blot (WB). GTX107967

Cat. No. GTX107967

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all UGP2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

Positive Control

HeLaS3

Predict Reactivity

Rat, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UGP2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038207

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UDP-glucose pyrophosphorylase 2 , UDPG , UDPGP , UDPGP2 , UGP1 , UGPP1 , UGPP2 , pHC379

Cellular Localization

Cytoplasm

Background

The enzyme encoded by this gene is an important intermediary in mammalian carbohydrate interconversions. It transfers a glucose moiety from glucose-1-phosphate to MgUTP and forms UDP-glucose and MgPPi. In liver and muscle tissue, UDP-glucose is a direct precursor of glycogen; in lactating mammary gland it is converted to UDP-galactose which is then converted to lactose. The eukaryotic enzyme has no significant sequence similarity to the prokaryotic enzyme. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967

GTX107967 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using UGP2(GTX107967) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967

GTX107967 IHC-P Image

UGP2 antibody [N3C3-2] detects UGP2 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UGP2 antibody [N3C3-2] (GTX107967) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UGP2 antibody [N3C3-2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107967

GTX107967 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using UGP2(GTX107967) antibody at 1:200 dilution.

Anti-UGP2 antibody [N3C3-2] used in Western Blot (WB). GTX107967

GTX107967 WB Image

Sample (30 ug of whole cell lysate)
A: Hela S3
7.5% SDS PAGE
GTX107967 diluted at 1:1000

Anti-UGP2 antibody [N3C3-2] used in IHC (Paraffin sections) (IHC-P). GTX107967

GTX107967 IHC-P Image

UGP2 antibody [N3C3-2] detects UGP2 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
UGP2 antibody [N3C3-2] (GTX107967) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UGP2 antibody [N3C3-2] used in Western Blot (WB). GTX107967

GTX107967 WB Image

KO/KD

The data was published in the journal Elife in 2016.PMID: 26981769

REFERENCE

Application Reference

Gehre L et al. Elife 2016; 5 : e12552 Sequestration of host metabolism by an intracellular pathogen.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169