Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:5000-1:20000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
rat liver , mouse liver , human liver
Predict Reactivity
Sheep, Bovine, Cat, Rhesus Monkey(>80% identity)
Form
Liquid
Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.93 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UGT1A. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11179114
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
BILIQTL1 antibody , GNT1 antibody , HUG-BR1 antibody , UDPGT antibody , UDPGT 1-1 antibody , UGT1 antibody , UGT1A antibody , UGT1A1 antibody , UDP-glucuronosyltransferase 1A1 antibody , UDP glycosyltransferase 1 family , polypeptide A1 antibody , bilirubin-specific UDPGT isozyme 1 antibody , UGT1.1 antibody , UDP-glucuronosyltransferase 1-1 antibody , UGT-1A antibody , bilirubin UDP-glucuronosyltransferase isozyme 1 antibody , UGT1*1 antibody , bilirubin UDP-glucuronosyltransferase 1-1 antibody , UDP-glucuronosyltransferase 1-A antibody , UGT1-01 antibody , UDP glucuronosyltransferase 1 family , polypeptide A1 antibody
Cellular Localization
Microsome , Endoplasmic reticulum membrane; Single-pass membrane protein
Background
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq]
Research Area
