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UHMK1 antibody [2H4]

Overexpression
Anti-UHMK1 antibody [2H4] used in Western Blot (WB). GTX83438
Anti-UHMK1 antibody [2H4] used in IHC (Paraffin sections) (IHC-P). GTX83438
Anti-UHMK1 antibody [2H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83438
Anti-UHMK1 antibody [2H4] used in Flow cytometry (FACS). GTX83438
Anti-UHMK1 antibody [2H4] used in IHC (Paraffin sections) (IHC-P). GTX83438
Anti-UHMK1 antibody [2H4] used in Western Blot (WB). GTX83438

Cat. No. GTX83438

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2H4

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Dog
Package
100 μl ($519)
See all UHMK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:150
FACS 1:100
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.82 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human UHMK1 (NP_787062) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10732116

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

U2AF homology motif kinase 1 , KIS , KIST , P-CIP2

Cellular Localization

Nucleus

Background

Upon serum stimulation, phosphorylates CDKN1B/p27Kip1, thus controlling CDKN1B subcellular location and cell cycle progression in G1 phase. May be involved in trafficking and/or processing of RNA.

Database

Research Area

DATA IMAGES

Anti-UHMK1 antibody [2H4] used in Western Blot (WB). GTX83438

GTX83438 WB Image

WB analysis of various cell lines using GTX83438 UHMK1 antibody [2H4].
Loading : 35 ug per lane

Anti-UHMK1 antibody [2H4] used in IHC (Paraffin sections) (IHC-P). GTX83438

GTX83438 IHC-P Image

IHC-P analysis of human lung carcinoma tissue using GTX83438 UHMK1 antibody [2H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-UHMK1 antibody [2H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83438

GTX83438 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with UHMK1 plasmid using GTX83438 UHMK1 antibody [2H4].

Anti-UHMK1 antibody [2H4] used in Flow cytometry (FACS). GTX83438

GTX83438 FACS Image

FACS analysis of HeLa cells using GTX83438 UHMK1 antibody [2H4].
Red : Primary antibody
Blue : Negative control antibody

Anti-UHMK1 antibody [2H4] used in IHC (Paraffin sections) (IHC-P). GTX83438

GTX83438 IHC-P Image

IHC-P analysis of human liver tissue using GTX83438 UHMK1 antibody [2H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.

Anti-UHMK1 antibody [2H4] used in Western Blot (WB). GTX83438

GTX83438 WB Image

Overexpression

WB analysis of HEK293T cells transfected with UHMK1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX83438 UHMK1 antibody [2H4].
Loading : 5 ug per lane

REFERENCE

Application Reference

Chu YD et al. Cancers (Basel) 2020; 12 (6) COX5B-Mediated Bioenergetic Alteration Regulates Tumor Growth and Migration by Modulating AMPK-UHMK1-ERK Cascade in Hepatoma.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519