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UHRF1 antibody [HL2287]

Recombinant antibody KO/KD Orthogonal
Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342
Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342
Anti-UHRF1 antibody [HL2287] used in IHC (Paraffin sections) (IHC-P). GTX638342
Anti-UHRF1 antibody [HL2287] used in IHC (Paraffin sections) (IHC-P). GTX638342
Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342

Cat. No. GTX638342

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2287

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all UHRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

90 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human UHRF1.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ubiquitin like with PHD and ring finger domains 1 , ICBP90 , Np95 , RNF106 , TDRD22 , hNP95 , hUHRF1 , huNp95

Cellular Localization

Nucleus

Background

This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]

Database

Research Area

DATA IMAGES

Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342

GTX638342 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342

GTX638342 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-UHRF1 antibody [HL2287] used in IHC (Paraffin sections) (IHC-P). GTX638342

GTX638342 IHC-P Image

UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
UHRF1 stained by UHRF1 antibody [HL2287] (GTX638342) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UHRF1 antibody [HL2287] used in IHC (Paraffin sections) (IHC-P). GTX638342

GTX638342 IHC-P Image

UHRF1 antibody [HL2287] detects UHRF1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
UHRF1 stained by UHRF1 antibody [HL2287] (GTX638342) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UHRF1 antibody [HL2287] used in Western Blot (WB). GTX638342

GTX638342 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with UHRF1 antibody [HL2287] (GTX638342) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations