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ULK1 antibody

Anti-ULK1 antibody used in Western Blot (WB). GTX132669
Anti-ULK1 antibody used in Western Blot (WB). GTX132669

Cat No. GTX132669

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

Jurkat , IMR32 , SK-N-AS , S-tagged ULK1-transfected 293T

Predict Reactivity

Mouse(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.236 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ULK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

unc-51 like autophagy activating kinase 1,ATG1,ATG1A,UNC51,Unc51.1,hATG1

Background

Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.

Database

Research Area

DATA IMAGES
Anti-ULK1 antibody used in Western Blot (WB). GTX132669

GTX132669 WB Image

Various whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000.

Anti-ULK1 antibody used in Western Blot (WB). GTX132669

GTX132669 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
There are currently no references for ULK1 antibody (GTX132669). Be the first to share your publications with this product.
REVIEW

ULK1 antibody Cat No. GTX132669

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 31-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169