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ULK1 antibody

Overexpression
Anti-ULK1 antibody used in Western Blot (WB). GTX132669
Anti-ULK1 antibody used in Western Blot (WB). GTX132669

Cat. No. GTX132669

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ULK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

Jurkat , IMR32 , SK-N-AS , S-tagged ULK1-transfected 293T

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ULK1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886706

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-51 like autophagy activating kinase 1 , ATG1 , ATG1A , UNC51 , Unc51.1 , hATG1

Background

Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.

Database

Research Area

DATA IMAGES

Anti-ULK1 antibody used in Western Blot (WB). GTX132669

GTX132669 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ULK1 antibody used in Western Blot (WB). GTX132669

GTX132669 WB Image

Various whole cell extracts (60 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 antibody (GTX132669) diluted at 1:1000.

REFERENCE

Application Reference

Li Z et al. Autophagy 2021; 17 (4) : 1054-1056 Phospho-regulation and function of ULK1-ATG13 during the cell cycle.
Submit a Reference

REVIEW

ULK1 antibody Cat. No. GTX132669

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ULK1 antibody used in Western Blot (WB). GTX132669
Date : Anonymous submitted on 31-Oct-2017
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 20 μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169