GeneTex
United States (US)

ULK1 (phospho Ser555) antibody

GTX132660_42543_20161110_WB_CCCP_18121410_987.jpg

Cat No. GTX132660

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

MCF-7 , MCF-7 (100 μM CCCP treatment for 2 hr)

Predict Reactivity

Mouse, Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.13 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues around human ULK1 (phospho Ser555). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Unc-51 Like Autophagy Activating Kinase 1,Atg1,Atg1A,Unc51,Unc51.1,Hatg1,Ulk1

Background

Involved in autophagy. Required for autophagosome formation (By similarity). Target of the TOR kinase signaling pathway that regulates autophagy through the control of phosphorylation status of ATG13/KIAA0652 and ULK1, and the regulation of the ATG13-ULK1-RB1CC1 complex (By similarity). Phosphorylates ATG13/KIAA0652. Involved in axon growth (By similarity). Plays an essential role in neurite extension of cerebellar granule cells.

Database

Research Area

DATA IMAGES
GTX132660_42543_20161110_WB_CCCP_18121410_987.jpg

GTX132660 WB Image

Untreated (–) and treated (+) MCF-7 whole cell extracts (60 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK1 (phospho Ser555) antibody (GTX132660) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169