Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ULK2(GTX111476) antibody at 1:200 dilution.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ULK2(GTX111476) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

ULK2 antibody [C2C3], C-term detects ULK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min