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ULK2 antibody [C2C3], C-term

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111476
Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

Cat. No. GTX111476

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

NT2D1 , Neuro2A , NIH-3T3 , Raw264.7 , C2C12

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ULK2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952511

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-51 like autophagy activating kinase 2 , ATG1B , Unc51.2

Background

This gene encodes a protein that is similar to a serine/threonine kinase in C. elegans which is involved in axonal elongation. The structure of this protein is similar to the C. elegans protein in that both proteins have an N-terminal kinase domain, a central proline/serine rich (PS) domain, and a C-terminal (C) domain. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

GTX111476 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

GTX111476 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

GTX111476 WB Image

ULK2 antibody [C2C3], C-term detects ULK2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ULK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111476

GTX111476 ICC/IF Image

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-ULK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111476

GTX111476 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ULK2(GTX111476) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

GTX111476 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ULK2 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111476

GTX111476 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ULK2(GTX111476) antibody at 1:200 dilution.

Anti-ULK2 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX111476

GTX111476 IHC-P Image

ULK2 antibody [C2C3], C-term detects ULK2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ULK2 stained by ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ULK2 antibody [C2C3], C-term used in Western Blot (WB). GTX111476

GTX111476 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ULK2 antibody [C2C3], C-term (GTX111476) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169