GeneTex
United States (US)

UMPS antibody

GTX114872_40240_IFA_18121410_849.jpg
GTX114872_WB_18121410_939.jpg
GTX114872_40240_IHC_18121410_475.jpg

Cat No. GTX114872

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

HeLa , HCT116

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UMPS. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Uridine Monophosphate Synthetase,Oprt,Umps

Background

This gene encodes a uridine 5'-monophosphate synthase. The encoded protein is a bifunctional enzyme that catalyzes the final two steps of the de novo pyrimidine biosynthetic pathway. The first reaction is carried out by the N-terminal enzyme orotate phosphoribosyltransferase which converts orotic acid to orotidine-5'-monophosphate. The terminal reaction is carried out by the C-terminal enzyme OMP decarboxylase which converts orotidine-5'-monophosphate to uridine monophosphate. Defects in this gene are the cause of hereditary orotic aciduria. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

DATA IMAGES
GTX114872_40240_IFA_18121410_849.jpg

GTX114872 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using UMPS(GTX114872) antibody at 1:200 dilution.

GTX114872_WB_18121410_939.jpg

GTX114872 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
10% SDS PAGE
GTX114872 diluted at 1:1000

GTX114872_40240_IHC_18121410_475.jpg

GTX114872 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using UMPS(GTX114872) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169