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UNC13B antibody [N2N3]

Anti-UNC13B antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX111244
Anti-UNC13B antibody [N2N3] used in Western Blot (WB). GTX111244
Anti-UNC13B antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111244

Cat. No. GTX111244

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all UNC13B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

181 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UNC13B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952517

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-13 homolog B , MUNC13 , UNC13 , Unc13h2

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Cell membrane , Cell junction , synapse

Background

This gene is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UNC13B antibody [N2N3] used in IHC (Paraffin sections) (IHC-P). GTX111244

GTX111244 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using UNC13B(GTX111244) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-UNC13B antibody [N2N3] used in Western Blot (WB). GTX111244

GTX111244 WB Image

Sample (30 ug of whole cell lysate)
A: Hela
5% SDS PAGE
GTX111244 diluted at 1:5000

Anti-UNC13B antibody [N2N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111244

GTX111244 ICC/IF Image

UNC13B antibody [N2N3] detects UNC13B protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: UNC13B stained by UNC13B antibody [N2N3] (GTX111244) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169