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UNC45B antibody, Internal

Anti-UNC45B antibody, Internal used in Western Blot (WB). GTX88214

Cat. No. GTX88214

Reference 1

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all UNC45B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

104 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88214-PEP).

Product Note

This antibody is expected to recognise both reported isoforms (NP_775259.1and NP_001028748.1).

Predict Reactivity

Mouse, Rat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-RSDKLRQKIFKER, from the internal region of the protein sequence according to NP_775259.1; NP_001028748.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

unc-45 myosin chaperone B , CMYA4 , CTRCT43 , SMUNC45 , UNC-45B , UNC45

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Database

Research Area

DATA IMAGES

Anti-UNC45B antibody, Internal used in Western Blot (WB). GTX88214

GTX88214 WB Image

WB analysis of human skeletal muscle lysate using GTX88214 UNC45B antibody, Internal.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

SH Lu et al. Stem Cell Reports 2023; S2213-6711 (23) : 00184-4 Cardiac myofibrillogenesis is spatiotemporally modulated by the molecular chaperone UNC45B
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399