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UNG antibody

Anti-UNG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113860
Anti-UNG antibody used in Western Blot (WB). GTX113860

Cat. No. GTX113860

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, Multiplexing

Reactivity

Human
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Multiplexing Assay dependent
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UNG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620655

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

uracil DNA glycosylase , DGU , HIGM4 , HIGM5 , UDG , UNG1 , UNG15 , UNG2

Cellular Localization

Isoform 1: Mitochondrion , Isoform 2: Nucleus

Background

This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UNG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113860

GTX113860 ICC/IF Image

UNG antibody detects UNG protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: UNG protein stained by UNG antibody (GTX113860) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-UNG antibody used in Western Blot (WB). GTX113860

GTX113860 WB Image

UNG antibody detects UNG protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of UNG shRNA#1-transfected HeLa cells
C. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells
11 % SDS-PAGE
UNG antibody (GTX113860) dilution: 1:1000

REFERENCE

REVIEW

UNG antibody Cat. No. GTX113860

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A. 30 ug HeLa whole cell lysate/extract, B. 30 ug whole cell lysate/extract of UNG shRNA#1-transfected HeLa cells, C. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169