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UNG antibody

Anti-UNG antibody used in Western Blot (WB). GTX113860
Anti-UNG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113860

Cat No. GTX113860

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

35 kDa. ( Note )

Positive Control

HeLa , HeLa transfect shUNG #1 , HeLa transfect shUNG #2

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Xenopus laevis, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.71 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UNG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620655

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

uracil DNA glycosylase , DGU , HIGM4 , HIGM5 , UDG , UNG1 , UNG15 , UNG2

Cellular Localization

Isoform 1: Mitochondrion , Isoform 2: Nucleus

Background

This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by eliminating uracil from DNA molecules by cleaving the N-glycosylic bond and initiating the base-excision repair (BER) pathway. Uracil bases occur from cytosine deamination or misincorporation of dUMP residues. Alternative promoter usage and splicing of this gene leads to two different isoforms: the mitochondrial UNG1 and the nuclear UNG2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-UNG antibody used in Western Blot (WB). GTX113860

GTX113860 WB Image

UNG antibody detects UNG protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg whole cell lysate/extract of UNG shRNA#1-transfected HeLa cells
C. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells
11 % SDS-PAGE
UNG antibody (GTX113860) dilution: 1:1000

Anti-UNG antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113860

GTX113860 ICC/IF Image

UNG antibody detects UNG protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37ºC for 30 min.
Green: UNG protein stained by UNG antibody (GTX113860) diluted at 1:500.
Blue: Hoechst 33342 staining.

REFERENCE

REVIEW

UNG antibody Cat No. GTX113860

Rating ( Average 4 based on 1 users reviews)
Application
Western Blot(WB) ( Average 4 based on 1 users reviews)
Date : Anonymous submitted on 19-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa cells
Amount used : 30μg
Blocking : 5% milk in TBS, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : A. 30 ug HeLa whole cell lysate/extract, B. 30 ug whole cell lysate/extract of UNG shRNA#1-transfected HeLa cells, C. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169