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UPF3B antibody, N-term

Anti-UPF3B antibody, N-term used in Western Blot (WB). GTX47258
Anti-UPF3B antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47258

Cat. No. GTX47258

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all UPF3B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Jurkat

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human UPF3B

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

UPF3B regulator of nonsense mediated mRNA decay , HUPF3B , MRX62 , MRXS14 , RENT3B , UPF3BP1 , UPF3BP2 , UPF3BP3 , UPF3X , Upf3p-X

Cellular Localization

Nucleus,Cytoplasm

Background

This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-UPF3B antibody, N-term used in Western Blot (WB). GTX47258

GTX47258 WB Image

WB analysis of Jurkat cells using GTX47258 UPF3B antibody at 1.25μg/ml.
Lane A : marker
Lane B : Jurkat cells

Anti-UPF3B antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47258

GTX47258 IHC-P Image

IHC-P analysis of human stomach tissue using GTX47258 UPF3B antibody at 4.0-8.0μg/ml.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399