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UPRT antibody

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370
Anti-UPRT antibody used in Western Blot (WB). GTX120370
Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

Cat No. GTX120370

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

A549

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human UPRT. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10732543

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

uracil phosphoribosyltransferase homolog , FUR1 , UPP

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes uracil phosphoribosyltransferase, which catalyzes the conversion of uracil and 5-phosphoribosyl-1-R-diphosphate to uridine monophosphate (UMP). This reaction is an important part of nucleotide metabolism, specifically the pyrimidine salvage pathway. The enzyme localizes to the nucleus and cytoplasm. The protein is a potential target for rational design of drugs to treat parasitic infections and cancer. [provided by RefSeq]

Database

DATA IMAGES

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

GTX120370 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using UPRT(GTX120370) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-UPRT antibody used in Western Blot (WB). GTX120370

GTX120370 WB Image

Sample (30 ug of whole cell lysate)
A: A549
12% SDS PAGE
GTX120370 diluted at 1:500

Anti-UPRT antibody used in IHC (Paraffin sections) (IHC-P). GTX120370

GTX120370 IHC-P Image

UPRT antibody detects UPRT protein at cytosol and nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
UPRT antibody (GTX120370) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169