APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888211
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ubiquinol-cytochrome c reductase core protein 1 , D3S3191 , QCR1 , UQCR1
Cellular Localization
Mitochondrion inner membrane
Background
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
Database
Research Area
DATA IMAGES
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/templates/default/js/lightbox/images/loading.gif "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 ICC/IF Image
UQCRC1 antibody [GT139] detects UQCRC1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.
Green: UQCRC1 protein stained by UQCRC1 antibody [GT139] (GTX630413) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139] (GTX630413) diluted at 1:500.
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REFERENCE
There are currently no references for UQCRC1 antibody [GT139] (GTX630413). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UQCRC1 antibody [GT139] (GTX630413). Be the first to share your experience with this product.
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_M_brain_w_23061202_363.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_R_brain_w_23061202_693.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_20150821_IFA_w_23061202_955.webp "Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_w_23061202_132.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_20161103_WB_shRNA_watermark_w_23061202_995.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_M_brain_w_23061202_363.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_R_brain_w_23061202_693.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_20150821_IFA_w_23061202_955.webp "Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_w_23061202_132.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_20161103_WB_shRNA_watermark_w_23061202_995.webp "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")