APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , 293T , A431 , HeLa , HepG2
Predict Reactivity
Bovine, Chimpanzee, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human UQCRC1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888211
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
ubiquinol-cytochrome c reductase core protein 1 , D3S3191 , QCR1 , UQCR1
Cellular Localization
Mitochondrion inner membrane
Background
This is a component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is part of the mitochondrial respiratory chain. This protein may mediate formation of the complex between cytochromes c and c1.
Database
Research Area
DATA IMAGES
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/templates/default/js/lightbox/images/loading.gif "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with UQCRC1 antibody [GT139] (GTX630413) diluted at 1:500.
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 50 μg mouse brain lysate/extract
7.5 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
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GTX630413 ICC/IF Image
UQCRC1 antibody [GT139] detects UQCRC1 protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature.
Green: UQCRC1 protein stained by UQCRC1 antibody [GT139] (GTX630413) diluted at 1:100.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
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GTX630413 WB Image
UQCRC1 antibody [GT139] detects UQCRC1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
UQCRC1 antibody [GT139] (GTX630413) dilution: 1:1000
|
REFERENCE
There are currently no references for UQCRC1 antibody [GT139] (GTX630413). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for UQCRC1 antibody [GT139] (GTX630413). Be the first to share your experience with this product.
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_R_brain_18121411_905.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_20161103_WB_shRNA_watermark_18121411_387.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_M_brain_18121411_612.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_20150821_IFA_18121411_898.jpg "Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/small/GTX630413/GTX630413_41540_WB_18121411_211.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_R_brain_18121411_905.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_20161103_WB_shRNA_watermark_18121411_387.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_M_brain_18121411_612.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_20150821_IFA_18121411_898.jpg "Anti-UQCRC1 antibody [GT139] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630413")
![Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413](/upload/website/prouct_img/normal/GTX630413/GTX630413_41540_WB_18121411_211.jpg "Anti-UQCRC1 antibody [GT139] used in Western Blot (WB). GTX630413")